Quercetin-Supplemented Diet Modulates Aluminium Chloride-Induced Neurotoxicity in Fruit Flies
DOI:
https://doi.org/10.26437.ajar.03.2022.6Abstract
Purpose: Neurotoxicity refers to the potential of a chemical substance, biological or physical agents to induce structural or functional defects in nerve tissue of the brain or peripheral nervous system. This study examined the therapeutic effect of dietary inclusion of Quercetin on Aluminium chloride-induced neurotoxicity in wild-type Fruit flies (Drosophila melanogaster).
Design/ Methodology/ approach: Flies were fed with diet supplements with AlCl3 (40mM) and Quercetin (0.1 and 1.0%) and counted daily throughout their life span. Flies were also raised on a diet supplemented with AlCl3 (40mM) and Quercetin (0.1 and 1.0%) for seven days. Flies were subsequently homogenized, and the activity of Acetylcholinesterase (AChE), Butyrylcholinesterase (BChE), Monoamine Oxidase (MAO), Catalase, and Glutathione-S-transferase (GST) were assayed.
Findings: Regarding the number of days required to reach 50% mortality, dietary inclusions of AlCl3 (40mM) reduced the life span of D.melanogaster more significantly than control flies. Also, Quercetin's 0.1 and 1.0% dietary inclusions significantly reduced AChE, BChE, and Monoamine Oxidase activity compared to the control.
Research Limitation/ Implications: The study focus on the dietary inclusion of Quercetin on Aluminium chloride-induced neurotoxicity in wild-type Fruit flies.
Practical Implication: There was a significant increase in the catalytic activities of GST and catalase in flies fed diet supplemented with 0.1 and 1.0% of Quercetin. However, dietary inclusion of both 0.1 and 1.0% of Quercetin seems tolerable as there was a less significant reduction in life span with a substantial effect on AChE, BChE, MAO activities, and its antioxidant activities compared to control flies.
Originality/ Value: The outcome of this research established that dietary inclusion of 40mM AlCl3 reduced the life span of D.melanogaster. In contrast, dietary inclusion of 0.1 and 1.0% of Quercetin produced some adaptive responses associated with reduction of neurotoxicity and elevated activities of some antioxidant enzymes.
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